Well I checked out PIE and TPA and I don't seem to find any air carriers that seem to base their operations there, I don't know Orlando (never lived there, and I avoid Class Bs like a cold) that well so I leave that to someone else.
Gulfstream Airlines has a pilot base in Tampa.
Air Midwest has bases in Orlando and Tampa.
Southwest Airlines has a pilot base in Orlando.
Comair had a base in Orlando, but I think it no longer existed after the strike.
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